The rat model of ICH was built up by intracerebral injection of collagenase IV.
mrats were sacrificed at 30 min,l h,4 h,l d,3 d,and 7 d after ICH.The brains
were removed,and the tissues from cortex and striatum were separated,and the
protein was extarcted at 4℃.Western blot Was used to analyze the alteration of
roTOR and its main downstreams,